Passive ultrasonic irrigation in the presence of a low concentration of hydrogen peroxide enhances hydroxyl radical generation and bactericidal effect against Enterococcus faecalis.

نویسندگان

  • Yoshimi Kobayashi
  • Makoto Hayashi
  • Fumihiko Yoshino
  • Muneaki Tamura
  • Ayaka Yoshida
  • Haruna Ibi
  • Masaichi-Chang-il Lee
  • Kuniyasu Ochiai
  • Bunnai Ogiso
چکیده

Chemomechanical procedures can be used to eliminate bacteria from root canals. However, detectable bacteria sometimes remain because of the complexity of the root canal system. Endodontic passive ultrasonic irrigation (PUI) with hydrogen peroxide (H2O2) may be a promising option for increasing bactericidal hydroxyl radical (HO•) generation. In this in vitro experiment, we examined the effects of HO• generated using PUI and a low concentration of H2O2. An ultrasonic tip was submerged in 0.45 mol/L (1.5%) H2O2 in a microfuge tube. H2O2 was activated by an ultrasonic unit, the tip of which was kept centered in the tube, to mimic PUI. HO• generation was detected by electron spin resonance spectroscopy. An Enterococcus faecalis suspension in H2O2 was then preparedand activated as described above. Bactericidal effects were assessed by viable counting. Two-way analysis of variance and Tukey's test were used to assess the statistical significance of differences among groups (P < 0.05). HO• generation and bactericidal activity were significantly increased by PUI in H2O2 in a time-dependent manner and were significantly higher than with H2O2 alone or with PUI in a Tris-HCl suspension. These results suggest that PUI in the presence of a low H2O2 concentration is a promising new disinfection strategy.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Bactericidal effect of hydroxyl radicals generated from a low concentration hydrogen peroxide with ultrasound in endodontic treatment

One approach to enhance the disinfection of root canals in endodontic treatment is ultrasonic irrigation with sodium hypochlorite. Reactive oxygen species, such as hydroxyl radical, are generated by biological defense systems to kill invading bacteria. Ultrasonic irrigation with hydrogen peroxide may be a promising option to increase hydroxyl radical generation. We examined the bactericidal eff...

متن کامل

Photolysis of hydrogen peroxide, an effective disinfection system via hydroxyl radical formation.

The relationship between the amount of hydroxyl radicals generated by photolysis of H(2)O(2) and bactericidal activity was examined. H(2)O(2) (1 M) was irradiated with laser light at a wavelength of 405 nm to generate hydroxyl radicals. Electron spin resonance spin trapping analysis showed that the amount of hydroxyl radicals produced increased with the irradiation time. Four species of pathoge...

متن کامل

Effect of Arginine, Protamine, and Aqueous Extracts of Green Tea and Aloe Vera Against Enterococcus faecalis

Background and Aim: Because of complexity in root canals, irrigating solutions are needed in addition to mechanical instrumentation for thorough cleansing of the root canal system. This in-vitro study was designed to determine the inhibitory effect of arginine, protamine, and aqueous extracts of green tea and aloe vera against Enterococcus faecalis (E. faecalis), which causes endodontic failure...

متن کامل

Kinetic study of unsaturated ketones epoxidation with hydrogen peroxide through the inverse phase transfer catalysis and effect of ultrasonic waves in this epoxidation

Kinetic study of epoxidation of unsaturated ketone of mesityl oxide was studied by usingHydrogen peroxide in the presence of dodecyltrimethyl ammonium bromide (DTAB) as aninverse phase transfer catalyst. The reaction was carried out in the two-phase media of waterheptanewith 1:1 ratio in 25 °C. It was found that the order of reaction for mesityl oxide forketone concentration in the range of 0.0...

متن کامل

Sonochemical degradation of azithromycin in aqueous solution

Background: The presence of pharmaceutical substances and their residual in water resources is an important environmental concern. Azithromycin, an antibiotic that is used for the treatment of infectious diseases, is a pollutant agent in the wastewater. The aim of this study was to investigate azithromycin degradation in aqueous solution through ultrasonic process in the presence of zinc oxide ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of oral science

دوره 56 1  شماره 

صفحات  -

تاریخ انتشار 2014